Structural energetics of cold sensitivity

Science

Structural Energetics Of Cold Sensitivity

By Nature

Cloning, cell culture and protein expression N-terminally fused mEGFP constructs of TRPM8 were obtained using Gibson assembly to sub-clone TRPM8 genes into a pFastBac1 vector. Mutagenesis was carried out by either PCR with mutagenic primers, or by Gi ...Read more